WERF Report Pathogens and Indicators (Project 00-HHE-2A-C)
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Available as an ebook
Please purchase via www.iwaponline.comOpens in new window
Also part of Water Intelligence Online Digital Reference Library
Standard ePrice: £29.00
+ VAT
This title belongs to WERF Research Report Series.
This report on Overcoming Molecular Sample Processing Limitations is split into three sections:
(a) New Platform Technologies
Rapid, low-cost extraction of DNA from bacterial cultures and colonies was investigated using four reagents: Instagene matrix (Bio-Rad, Hercules, CA); Isocode paper (Schleicher and Schuell, Keene, NH); PrepMan (Applied Biosystems, Foster City, CA); and Xtra Amp tubes (Ansys Diagnostics, Lake Forest, CA).
(b) RNA and DNA Extraction Strategies
The purpose of this study was 1) to develop standardized protocols for the rapid (less than 8 hours) quantitative PCR detection of total intact Cryptosporidium and potentially infectious enteroviruses in wastewater samples, 2) to determine the detection sensitivities of the methods using seeded wastewater samples, 3) to provide statistical evaluation for the determination of reproducibility, accuracy of quantitation, and variability of the methods, and 4) to investigate the feasibility of the developed methods using unseeded wastewater samples. Concentration methods for two different volumes of Cryptosporidium and enterovirus samples were evaluated. This allowed a comparison of the methods based on their ease of use, cost and performance.
(c) Fiber-Optic Biosensors
This study investigated the use of a commercially available fiber optic biosensor (Analyte 2000TM, Research International, Woodinville, WA) for near real-time detection of the pathogens E. coli O157:H7 and Salmonella typhimurium in wastewater.
The literature review described in this report is part of a larger research project to assess STU performance with respect to treatment of important wastewater constituents. The overall goal of...
The overall aim of this research was to evaluate membrane bioreactor (MBR) process designs to meet low effluent nitrogen and/or phosphorus concentrations from municipal wastewater treatment...
One of the largest contributions of GHG emissions from wastewater treatment facilities results from the energy used by the pumping regime of the lift stations. This...
Anaerobic/aerobic (AnA) and completely aerobic (CA) laboratory-scale sequencing batch reactors operating on an acetate- and casamino acids-based synthetic wastewater were used to...
The research team field-tested a draft 5-step protocol over the length of the project from May 2009 until October 2010 on XX number of complaints (20 from May 2009 to September 2010). The...
River Basin Restoration and Management is the result of two workshops that took place at the 4th IWA World Water Congress: The Restoration of Degraded River Basins and River Basin...
Although ingestion of high levels of arsenic is believed to cause certain cancers, estimates of cancer risk resulting from exposure to low levels of arsenic are the subject of considerable debate...
Direct potable reuse (DPR) is the planned introduction of recycled water either directly into a public water system or into a raw water supply immediately upstream of a water treatment plant. DPR...