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This title belongs to WERF Research Report Series.
This report on Overcoming Molecular Sample Processing Limitations is split into three sections:
(a) New Platform Technologies
Rapid, low-cost extraction of DNA from bacterial cultures and colonies was investigated using four reagents: Instagene matrix (Bio-Rad, Hercules, CA); Isocode paper (Schleicher and Schuell, Keene, NH); PrepMan (Applied Biosystems, Foster City, CA); and Xtra Amp tubes (Ansys Diagnostics, Lake Forest, CA).
(b) RNA and DNA Extraction Strategies
The purpose of this study was 1) to develop standardized protocols for the rapid (less than 8 hours) quantitative PCR detection of total intact Cryptosporidium and potentially infectious enteroviruses in wastewater samples, 2) to determine the detection sensitivities of the methods using seeded wastewater samples, 3) to provide statistical evaluation for the determination of reproducibility, accuracy of quantitation, and variability of the methods, and 4) to investigate the feasibility of the developed methods using unseeded wastewater samples. Concentration methods for two different volumes of Cryptosporidium and enterovirus samples were evaluated. This allowed a comparison of the methods based on their ease of use, cost and performance.
(c) Fiber-Optic Biosensors
This study investigated the use of a commercially available fiber optic biosensor (Analyte 2000TM, Research International, Woodinville, WA) for near real-time detection of the pathogens E. coli O157:H7 and Salmonella typhimurium in wastewater.
Research Roadmap: Towards an Economic Decision Methodology for Remaining Asset Life
This report is an output of the fourth research track (Track 4) of WERF’s strategic asset management research program ‘Asset Management Communication and Implementation’ (RFPP #06-SAM-1 CO). Track...
Validation of Rapid Methods for Enumeration of Markers for Human Sewage Contamination in Recreational Waters
Rapid methods of water quality assessment are essential for informing the public and officials of sewage-polluted water in a timely manner. Since fecal indicator bacteria can be contributed to the...
Application of a Dynamic Model to Assess Microbial Health Risks Associated with Beneficial Uses of Biosolids
Microbial risk assessment is a structured approach to estimating risks associated with environmental exposure to pathogens by using available environmental data.
In this report the...
Adapting Leading Practices and Associated Tools
The purpose of the demonstration of WERF Strategic Asset management (SAM) decision support tools is to provide users with insight into the efforts and impacts of applying the various asset...
Regenerative Sanitation: A New Paradigm For Sanitation 4.0
This book proposes Regenerative Sanitation as the next era of sanitation management and attempts to provide a foundation for the study of sanitation on the premise that sanitation is a complex and...
Nutrient Speciation and Refractory Compounds in Water Quality Models
Water quality modeling efforts are designed to provide an understanding of watershed conditions to support management efforts that include control of point and nonpoint sources (NPS). Nitrogen (N...
Mainstream Deammonification
This is an eBook available to WIO subscribers only.
Cryptosporidium Literature Review
This report provides a review of the literature on Cryptosporidium and helps to identify the findings and formulates the key research questions. It covers these central questions:
• What...