Standard price: £29.00

Currency:

Available as an ebook:
Please purchase via www.iwaponline.comOpens in new window

Also part of Water Intelligence Online Digital Reference Library

Standard ePrice: £29.00

+ VAT

Purchase ebook

Publication Date: 30/09/2004

Pages: 112

Binding: Paperback

ISBN13: 9781843397274

eISBN: 9781780404356

WERF Report 01-HHE-1

OverviewExpand or collapse me

Deoxyribonucleic acid (DNA) microarrays are widely used for differential expression studies and for detection of virulence genes in pure bacterial cultures. Their use in complex microbial samples, such as soil and wastewater, has been relatively less studied.

This report presents the results of a systematic effort to apply DNA microarrays to pathogen detection and to bacterial source tracking (BST) in wastewater. Parameters such as the method of DNA extraction from the samples, the type of immobilized probe (whether polymerase chain reaction [PCR] amplicons or oligonucleotides), the length and method of immobilization of oligonucleotides, the method of DNA labelling, the combination of PCR amplification with microarray hybridization and the choice of PCR targets have been optimized.
Results indicate that the combination of PCR followed by microarray hybridization can detect pathogens in wastewater samples down to a 0.1% detection limit. The use of microarrays for bacterial source tracking gave promising results on human samples, however the probes used in this study only provided signals for general indicators of fecal contamination when used on samples of animal origin.

The overall conclusion is that microarray technology has not yet reached the stage of routine use for microbiological analysis of wastewater.

This report demonstrates:

  • That the hybridization of total genomic DNA on microarrays has a high detection limit, of the order of 10^7 genomes;           
  • That the use of long oligonucleotides or PCR amplicons from 16S rDNA, or cpn60 probes has insufficient specificity to differentiate several important pathogens, especially within the Enterobacteriaceae family;           
  • That the use of short oligonucleotide immobilized probes coupled with PCR amplification of conserved genes, such as 16S rDNA, cpn60, or wecE, can detect pathogens in wastewater down to a 0.1% (DNA weight/weight) concentration; and           
  • The potential of DNA microarrays in BST, even if further research work remains necessary to achieve this goal.

 

Share this page:

The latest from @IWApublishing

Great exposure for this important report from our journal. Read more about development and hydropower… https://t.co/mKU5PdVKx0 11 hours 32 min ago
RT : Share your vision for water and peace! Water touches many aspects of our lives, while also fostering cooperation a… https://t.co/EvD7WZJTSr 16 hours 22 min ago
RT : Professional development opportunities through IWA, straight to your inbox https://t.co/AfbbMdD7NJ https://t.co/qicqA3KZ4R 3 days 10 hours ago