Understanding Viability of Pathogens During DisinfectionUnderstanding Viability of Pathogens During DisinfectionMunicipal wastewater is the largest reservoir of enterobacteria (total coliforms) and a host of other microorganisms. Wastewater also harbors a range of pathogenic, opportunistic pathogenic, and nonpathogenic species. The public health significance of this reservoir may depend upon the physiological status of the wastewater microbial community. Viability and culturability are integral aspects of bacterial physiology but their relationship in the context of wastewater processing and disinfection are poorly understood. While culturable microbes must be viable, viable organisms may not be culturable, but they can retain metabolic activity, membrane integrity, and the capacity to resume culturability (growth). The ability of an organism to be cultured can be a variable trait. Transient loss of culturability precipitated by disinfection would confound containment assessment and pathogen control. To better understand the relationship between coliform physiology and disinfection, culture-independent methods are necessary. Such methods can avoid culture-bias and the loss of information reflecting the in situ experience of resident organisms. While the relationship between viable but not culturable bacterial pathogens to public health risk is important, it remains poorly understood and outside the scope of the current study.
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